Make sure HEPA filters and a dual flask system is utilized as well as a secondary container to avoid any potential spill. Add disinfectant to the vessels daily and empty at the end of the working day.
An example of a suitable disinfectant is bleach at final concentration of at least 10% (use concentrated bleach and calculate volume necessary based on volume of the flask).
Vacuum System for Biological Materials
Vacuum lines must be protected by High Efficiency Particulate Air (HEPA) filters. A dual flask system should be utilized. Flasks should be stabilized within a vessel that can contain a potential spill. Flask should contain appropriate disinfectant to decontaminate waste and be emptied at the end of work that day.
Alternatively, vacuum canisters with automatic shut-off valves may be used.